Entry 1 sense Webster, 1913 Luciferase /(lo͞o*sĭf"ẽr*ās)/ · Lu·cif·er·ase · IPA 01 n. an enzyme which catalyzes the oxidation of luciferin, thereby producing bioluminescence. 1. an enzyme which catalyzes the oxidation of luciferin, thereby producing bioluminescence.(Biochem.)